Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10
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چکیده
منابع مشابه
Exploring the binding sites of the haloalkane dehalogenase DhlA from Xanthobacter autotrophicus GJ10.
The catalytic site of haloalkane dehalogenase DhlA is buried more than 10 A from the protein surface. While potential access channels to this site have been reported, the precise mechanism of substrate import and product export is still unconfirmed. We used computational methods to examine surface pockets and their putative roles in ligand access to and from the catalytic site. Computational so...
متن کاملDegradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10.
A bacterium that is able to utilize a number of halogenated short-chain hydrocarbons and halogenated carboxylic acids as sole carbon source for growth was identified as a strain of Xanthobacter autotrophicus. The organism constitutively produces two different dehalogenases. One enzyme is specific for halogenated alkanes, whereas the other, which is more heat stable and has a higher pH optimum, ...
متن کاملThree-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate.
The L-2-haloacid dehalogenase from the 1,2-dichloroethane degrading bacterium Xanthobacter autotrophicus GJ10 catalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Its crystal structure was solved by the method of multiple isomorphous replacement with incorporation of anomalous scattering information and solvent flattening,...
متن کاملA bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis.
The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihalo- alkanes, such as 1, 2-dichloroethane (DCE), to a halogenated alcohol and an inorganic halide (Janssen et al. 1994, Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolized by alcohol and aldehyde dehydrogenase activities, and by the product of the dhlB gene to a se...
متن کاملA pH-indicator-based screen for hydrolytic haloalkane dehalogenase.
1. Introduction Microbial hydrolytic haloalkane dehalogenases catalyze the cleavage of halogen-carbon bonds of a variety of aliphatic halogenated compounds, including a broad range of chlorinated (C 2 –C 6) and brominated (C 2 –C 8) alkanes, with water as the sole co-substrate, resulting in the production of halide ions, protons, and alcohols (1,2). Based primarily on substrate specificity and ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.163.2.635-639.1985